英文名稱    DOCK1    

中文名稱    細胞質分裂付出蛋白1抗體    

別    名    180 kDa protein downstream of CRK; Ced5; Dedicator of Cytokinesis 1; Dedicator of cytokinesis protein 1; DOCK 1; DOCK-1; DOCK 180; DOCK1; DOCK1_HUMAN; DOCK180.    

供應商     遠慕生物

研究領域    腫瘤  細胞生物  信號轉導  細胞凋亡  細胞自噬  G蛋白信號      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應    Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep,     

產品應用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）細胞質分裂付出蛋白1抗體 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    215kDa    

細胞定位    細胞漿 細胞膜     

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human DOCK1    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

細胞質分裂付出蛋白1抗體產品介紹    background:

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
Tissue specificity: Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.

Function:
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1. 

Subunit:
Interacts with the SH3 domains of CRK and NCK2 via multiple sites. Interacts with nucleotide-free RAC1 via its DHR-2 domain. Interacts with ELMO1, ELMO2 and probably ELMO3 via its SH3 domain. Interacts with RAC1 and BAI1. 

Subcellular Location:
Cytoplasm (Probable). Membrane (Probable). Note=Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate (Probable). 

Tissue Specificity:
Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.

Similarity:
Belongs to the DOCK family. 
Contains 1 DHR-1 domain. 
Contains 1 DHR-2 domain. 
Contains 1 SH3 domain. 

Database links:
UniProtKB/Swiss-Prot: Q14185.2